Journal of Separation Science-03 February 2018

Simultaneous determination and qualitative analysis of six types of components in Naoxintong capsule by miniaturized matrix solid-phase dispersion extraction coupled with ultra high-performance liquid chromatography with photodiode array detection and quadrupole time-of-flight mass spectrometry
期刊名：Journal of Separation Science
文献编号：DOI 10.1002/jssc.201701411
文献地址：https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201701411
发表日期：03 February 2018

Abstract

A simple and effective sample preparation process based on miniaturized matrix solid‐phase dispersion was developed for simultaneous determination of phenolic acids (gallic acid, chlorogenic acid, ferulic acid, 3,5‐dicaffeoylqunic acid, 1,5‐dicaffeoylqunic acid, rosmarinic acid, lithospermic acid, and salvianolic acid B), flavonoids (kaempferol‐3‐O‐rutinoside, calycosin, and formononetin), lactones (ligustilide and butyllidephthalide), monoterpenoids (paeoniflorin), phenanthraquinones (cryptotanshinone), and furans (5‐hydroxymethylfurfural) in Naoxintong capsule by ultra high‐performance liquid chromatography. The optimized condition was that 25 mg Naoxintong powder was blended homogeneously with 100 mg Florisil PR for 4 min. One milliliter of methanol/water (75:25, v/v) acidified by 0.05% formic acid was selected to elute all components. It was found that the recoveries of the six types of components ranged from 61.36 to 96.94%. The proposed miniaturized matrix solid‐phase dispersion coupled with ultra high‐performance liquid chromatography was successfully applied to simultaneous determination of the six types of components in Naoxintong capsules. The results demonstrated that the proposed miniaturized matrix solid‐phase dispersion coupled with ultra high‐performance liquid chromatography could be used as an environmentally friendly tool for the extraction and determination of multiple bioactive components in natural products.

Reference standards including gallic acid (GA), 5-hydroxymethylfurfural, chlorogenic acid, paeoniflorin, ferulic acid, 3,5-dicaffeoylqunic acid, 1,5-dicaffeoylqunic acid, kaempferol-3-O-rutinoside, rosmarinic acid, lithospermic acid, salvianolic acid B, calycosin, formononetin, ligustilide, butyllidephthalide, and cryptotanshinone were purchased from Chengdu Desite Biological Technology Co., Ltd (Chengdu, China)