摘要：目的 通过建立高粱泡叶药材中异槲皮素活性成分的薄层鉴别(TLC)及高效液相色谱分析方法(HPLC)测定槲皮素含量。方法 70%乙醇为溶剂超声提取高粱泡叶中异槲皮素,以乙酸乙酯∶甲酸∶水∶氨水(18∶2∶1∶0.5)为展开剂于聚酰胺薄膜上展开,3%三氯化铝乙醇溶液为显色剂,105℃烘3 min, 365 nm下检视。采用HPLC,以异槲皮素为对照品,色谱柱为Agilent SB-C18柱(5μm, 4.6 mm×150 mm),以乙腈为流动相(B),以水(含2%磷酸)为流动相(A),梯度洗脱,洗脱程序如下:0~2 min, 10.0%~15.0%B;2~22 min, 15.0%~19.5%B;22~26 min, 19.5%~21.0%B;26~30 min, 21.0%~20.0%B;30~35 min, 20.0%~10.0%B;35~45 min, 10.0%B。柱温30℃,检测波长360 nm,流速1.0 mL/min。结果 高粱泡叶中异槲皮素在紫外灯下出现明显斑点。异槲皮素2~20μL范围内呈良好的线性关系,相关系数(r)为1,平均回收率(n=9)分别为95%,99%,103%,RSD分别为1.0%,0.8%,0.6%。结论 本实验所建立的高梁泡叶药材异槲皮素含量测定方法简便快速、准确可行,通过对高粱泡叶薄层色谱及含量测定方法的建立,为后期高粱泡叶的应用开发奠定相关基础。
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