摘要：
目的:通过UPLC指纹图谱比较正品防己及其混伪品广防己的差异,并结合化学计量学方法为防己药材的鉴别提供依据。方法:选用ACQUITY UPLC HSS T3（100 mm×2.1 mm,1.8μm）色谱柱,以0.2%磷酸溶液-乙腈为流动相,梯度洗脱;流速为0.2 mL/min;检测波长为210 nm。结果:建立的指纹图谱共标定了18个共有峰,通过与对照品比对,指认了5个成分,分别为木兰箭毒碱、波尔定碱、木兰花碱、防己诺林碱、粉防己碱;22批正品防己的相似度均在0.940以上,混伪品的相似度均≤0.239。聚类分析、主成分分析、正交偏最小二乘判别分析的结果一致,26批防己、广防己样品聚为2类,并筛选出3个差异标志性成分。结论:该方法能有效区分防己及其混伪品,可为控制防己药材的质量提供参考。
…………
1 仪器与材料
1.2
材料 木兰箭毒碱(批号: DST200426-014)、波尔定碱(批号: DST200320-092)、木兰花碱(批号:DST190625-004)、防己诺林碱(批号: DST190701-005)、粉防已碱(批号:DST200801-004)对照品均购自成都德思特生物技术有限公司；
…………
Three-dimensional characteristic chromatogram by online comprehensive two-dimensional liquid chromatography: Application to the identification and differentiation of ginseng from herbal medicines to various Chinese patent medicines
A practical strategy enabling more reliable identification of ginsenosides from Panax quinquefolius flower by dimension-enhanced liquid chromatography/mass spectrometry and quantitative structure-retention relationship-based retention behavior prediction
Unveiling the chemical components variation of Sishen formula induced by different prescription ratios by the advanced liquid chromatography/mass spectrometry approaches
Machine learning prediction for constructing a universal multidimensional information library of Panax saponins (ginsenosides)
In vitro and in vivo metabolic activation and hepatotoxicity of chlorzoxazone mediated by CYP3A
A comprehensive strategy integrating metabolomics with DNA barcoding for discovery of combinatorial discriminatory quality markers: A case of Cimicifuga foetida and Cimicifuga dahuric
推荐阅读
热门阅读
结构类型
-->