摘要：[目的] 通过建立中药细辛水提物高效液相色谱（HPLC）指纹图谱，评价不同产地细辛药材水提物的质量差异。[方法] 采用ZORBAX Eclipse Plus C18柱（4.6 mm×250 mm，5 μm）；以0.1%甲酸水溶液（A）-乙腈（B）为流动相进行梯度洗脱洗；流速1mL/min；柱温：30 ℃；进样量：5 μL；检测波长为285 nm。建立10 批细辛药材水提物的指纹图谱，通过相似度分析并结合聚类分析（HCA）、偏最小二乘判别分析（PLS-DA）、正交偏最小二乘法-判别分析（OPLS-DA）及主成分分析（PCA）对细辛药材水提物进行质量评价。[结果] 建立的细辛药材水提物指纹图谱共标定了20 个共有峰，通过与对照品比对，指认出6 个化学成分，10 批样品的相似度均在0.908 以上。HCA、PLS-DA、OPLS-DA 及PCA 结果表明10 批细辛药材水提物之间存在一定的质量差异，这些结果可用于对不同产地的细辛药材水提物进行质量评价。[结论] HPLC 指纹图谱结合化学模式识别技术可系统、全面地评价细辛药材水提物的质量。对中药材细辛水提物进行研究可进一步完善对细辛药材的质量评价。
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1.2试药 对照品细辛脂素（批号：DST180425-014、马兜铃酸 A （批号：DST180413-02I芝麻脂素（批号: MUST -1706301、卡枯醇（批号：DST190325-028去甲乌药碱（批号：DST190402-154、槲皮素（批号：DST180521-028购于成都德思特生物科技有限公司。
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DOI：10.11656/j.issn.1673-9043.2022.02.24
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